Chicken assembly and gene annotation

Assembly

The Gallus_gallus-5.0 assembly of the chicken genome was released in December 2015 by the International Chicken Genome Consortium. It consists of 34 chromosomes, 1 linkage group and 15,411 unplaced scaffolds.

The genome assembly represented here corresponds to GenBank Assembly ID GCA_000002315.2

Other assemblies

Gene annotation

Gallus_gallus-5.0 was annotated using a standard Ensembl genebuild pipeline, incorporating RNASeq data (PRJEB12891) and PacBio long read data (PRJEB13248, PRJEB13246) provided by the Roslin Institute. The annotation process is described in the document below.

PacBio long read data set

Two tissue samples were sequenced using the PacBio long read sequencing technology, embryo and brain. Both sets were used to add UTR to gene models and as input source for our lincRNA discovery pipeline. The embryo set was sequenced using 5' and 3' capping, therefore all the sequences were considered as full length cDNAs and incorporated into the gene models.

RNASeq data set

In addition to the main set, we have predicted gene models for each tissue type using the RNA-Seq pipeline. We did a BLASTp of these models against UniProt proteins of vertabrate species with protein existence level 1 and 2 in order to confirm the open reading frame (ORF). The best BLAST hit is displayed as a transcript supporting evidence. The data was also used to add UTR to gene models.

The tissue-specific sets of transcript models built using our RNAseq pipeline are as follows:

TissueNumber of gene models
Breast muscle6132
Bursa8388
Caecal tonsil10421
Cerebellum8567
Duodenum8808
Gizzard fat8892
Harderian gland6544
Heart muscle7746
Ileum8309
Kidney7611
Left optic lobe7616
Liver8751
Lung6774
Ovary8112
Pancreas8271
Proventriculus8316
Skin1751
Spleen8232
Thymus7332
Thyroid7766
Trachea8987
Merged14200

More information

General information about this species can be found in Wikipedia.

Statistics

Summary

AssemblyGalgal4, INSDC Assembly GCA_000002315.2, Nov 2011
Base Pairs1,072,544,763
Golden Path Length1,046,932,099
Annotation methodFull genebuild
Genebuild startedDec 2011
Genebuild releasedApr 2013
Genebuild last updated/patchedDec 2013
Database version94.4

Gene counts

Coding genes15,508
Pseudogenes42
Gene transcripts17,954

Other

Genscan gene predictions40,572
Short Variants9,456,218

About this species